Proximity Ligation Assay (PLA)
نویسندگان
چکیده
منابع مشابه
Proximity ligation assay evaluates IDH1R132H presentation in gliomas.
For a targeted cancer vaccine to be effective, the antigen of interest needs to be naturally processed and presented on MHC by the target cell or an antigen-presenting cell (APC) in the tumor stroma. The presence of these characteristics is often assumed based on animal models, evaluation of antigen-overexpressing APCs in vitro, or assays of material-consuming immune precipitation from fresh so...
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Techniques for studying RNA-protein interactions have lagged behind those for DNA-protein complexes as a consequence of the complexities associated with working with RNA. Here we present a method for the modification of the existing In Situ Hybridisation-Proximity Ligation Assay (ISH-PLA) protocol to adapt it to the study of RNA regulation (rISH-PLA). As proof of principle we used the well-char...
متن کاملSH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR
BACKGROUND There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyros...
متن کاملDetecting individual extracellular vesicles using a multicolor in situ proximity ligation assay with flow cytometric readout
Flow cytometry is a powerful method for quantitative and qualitative analysis of individual cells. However, flow cytometric analysis of extracellular vesicles (EVs), and the proteins present on their surfaces has been hampered by the small size of the EVs - in particular for the smallest EVs, which can be as little as 40 nm in diameter, the limited number of antigens present, and their low refr...
متن کاملImproving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single mo...
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ژورنال
عنوان ژورنال: Current Protocols in Immunology
سال: 2018
ISSN: 1934-3671
DOI: 10.1002/cpim.58